Polarized secretion of androgen-binding protein and transferrin by Sertoli cells grown in a bicameral culture system

Sertoli cells in vivo are believed to secrete androgen-binding protein (ABP) as well as other proteins in a polarized manner, primarily into the adluminal (apical) compartment of the seminiferous tubules. It is now possible to examine polarized secretion by Sertoli cells in vitro by growing them in dual environment (bicameral) culture chambers such that there is a separation of the apical and basal compartments of the cells. Sertoli cells obtained from 17-day-old rats were grown in these chambers on Millipore filters coated with a reconstituted basement membrane matrix. Within 3 days a confluent epithelial sheet of Sertoli cells (30–40 μm in height) is established, and these cells are joined along their baso-lateral plasma membranes by typical Sertoli-Sertoli tight junctions. We have previously shown that this epithelial sheet of Sertoli cells establishes an electrical resistance, as well as a permeability barrier to small molecules, between the basal and apical compartments of the culture chamber. In the...