The relationship between ABCC4 expression and gastric cancer cell proliferation

Objective To observe the effect of ABCC4(ATP-binding cassette, sub-family C member 4) on gastric cancer cell line SGC-7901's proliferation and apoptosis. Methods SGC-7901 cell line were infected by the lentivirus carried the RNAi of ABCC4. were detected the proliferation and clone formation through CELLOMICS. Flow cytometry was applied to detect the cell cycle and apoptosis. Results The infection of SC C-7901 cell line with lentivirus carried the RNAi of ABCC4 alleviated the proliferation of cell line, changed the cell cycle, significantly decreased the cell number of Go/G1 (t=2.752,P＜0.05), significantly increased the cell number of G2/M (t=2.973, P＜0.05), at the same time significantly increased apoptosis (t= 3.068, P＜0.05), and inhibited the formation of cell clone (t = 3.534, P＜0.05). Conclusion The inhibition of gene ABCC4 can alleviate the malignant proliferation of SGC-7901 and produce its apoptosis. Key words: ABCC4;  Gastric cancer;  Cell proliferation;  Apoptosis