Pedobacter zeae sp. nov., an endophytic bacterium isolated from maize root.
2017
A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain, designated 22T, was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, PR China. The highest levels of 16S rRNA gene sequence similarity were found with respect to
Pedobacter suwonensis
15-52T (97.5 %),
Pedobacter terrae
DS-57T (97.1 %) and
Pedobacter alluvionis
NWER-II11T (97.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus
Pedobacter
. The isolate exhibited relatively low levels of DNA–DNA relatedness with respect to
P. suwonensis
DSM 18130T (21.3±2.0 %),
P. alluvionis
DSM 19624T (38.1±1.8 %) and
P. terrae
DSM 17933T (17.1±1.4 %). The DNA G+C content was 41.2±0.5 mol%. The major isoprenoid quinone was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids and one unidentified lipid. The major fatty acids were identified as iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 22T from the related species with high 16S rRNA gene sequence similarity,
P. suwonensis
DSM 18130T,
P. alluvionis
DSM 19624T and
P. terrae
DSM 17933T. Strain 22T represents a novel species within the genus
Pedobacter
, for which the name Pedobacter zeae sp. nov. is proposed, with the type strain 22T (=CGMCC 1.15287T=DSM 100774T).
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