In vitro non-enzymatic glycation and formation of browning products in the bovine lens α-crystallin
1990
Abstract Calf lens LMW α-crystallin was glycated by incubating with various sugars (glucose, glucose-6-P and ribose) for 21 days. All sugars induced disulfide formation, but ribose also produced higher molecular weight cross-linked species. The ribocated protein turned yellow in color and had a strong blue fluorescence ( Ex Em = 370 450 nm ) typical for a browning product. The chromophore of the browning product showed a new circular dichroism (CD) band at 320–330 nm. Conformational study indicated that the browning reaction destabilized protein and may play a significant role in protein aggregation and insolubilization.
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