Tobacco cultivar 6 malic rich set of methods Tissue Culture

The present invention discloses a method of malic rich tobacco cultivar 6 Tissue Culture, the tobacco enriched apple cultivar group Tissue Culture Method 6, by using the medium of MS + 6-BA0.5mg / L + IBA0. 1mg + PVP500mg / L initial culture medium, using MS + 6-BA0.5mg / L + IBA0.1mg / L subculture process using rooting medium 1 / 2MS + IBA1.0mg / L of rooting, transplanting with hardened pure sawdust; to achieve a rapid propagation of smoke rich apple varieties No. 6, not only to ensure a high survival rate, but also retains the excellent characteristics of the breed, providing convenient for the apple industry and the development of further expansion ways and means.