TGEV and porcine epidemic diarrhea virus detection primers, probes and detection kit

The present invention discloses a TGEV and porcine epidemic diarrhea virus detection primers, probes and detection kits. The detection primer and probe sequence table SEQIDNO: 1 to SEQIDNO: 6, in which the sequence SEQIDNO: 1 and SEQIDNO: 2, respectively, for the detection of TGEV sense primer and antisense primer sequence SEQIDNO: 3 to detect TGEV fluorescent probe sequence SEQIDNO: 4 and SEQIDNO: 5, respectively, for the detection of porcine epidemic diarrhea virus sense primer and antisense primer sequence SEQIDNO: 6 for the detection of porcine epidemic diarrhea virus fluorescent probes. The present invention also provides a TGEV and porcine epidemic diarrhea virus detection kit. The selected primers and probes specifically the present invention is very strong, total RNA virus in pigs diarrhea was extracted without first transcribed into cDNA, PCR cDNA synthesis and double strand first step, and one can detect two viruses ,Improve efficiency.