Spline-Based Multimodal Image Registration of 3D PLI Data of the Human Brain

Registration of high-resolution 3D polarized light images (PLI) to reference blockface images is necessary for the analysis of brain fiber structures. We present an automatic approach for the registration of high-resolution 3D PLI data of histological sections of a human brain that integrates automatic segmentation, rigid registration, and splinebased elastic registration. We have applied the approach to images of the temporal lobe of the human brain and assessed its accuracy and robustness.