[Chemoattractive effects of chemokine-like factor 1 on human arterial smooth muscle cells].

Objective:To investigate the chemotactic effect of chemokine-like factor 1(CKLF1)on human arterial smooth muscle cells(ASMCs).Methods:The recombinant eukaryotic expression vectors pEGFP-N1-CKLF1(test group) and pEGFP-N1(control group)were transiently transfected into 293T cells.The supernatants were harvested 72 h after transfection for bioactivity study.The chemotactic effect of CKLF1 on ASMCs were assayed by cellular chemotactic experiments.Results:ASMCs number migrated from the test and control groups diluted by none and 10-fold supernatants had statistical significance(114±4 vs 41±4,P0.05;74±4 vs 34±3,P0.01),but have no statistical significance(28±4 vs 25±5,P0.05;26±5 vs 23±5,P0.05) between the two groups diluted by 100-fold and 1 000-fold supernatants.When ASMCs were treated at different concentrations of 0 and 2 μg/L of pertussis toxin(PTX),the cell number migrated from the test and control groups diluted by 10-fold supernatants,they had statistical significance(74±4 vs 34±3,P0.01;45±3 vs 34±3,P0.01).And when ASMCs were treated at 10 μg/L of PTX,the cell number migrated from both groups diluted by 10-fold supernatants,they had no statistical significance(37±4 vs 34±3,P0.05).Conclusion:CKLF1 has significant chemotactic effects on ASMCs and such a CKLF1-induced chemotaxis could be inhibiteded by PTX at concentration of 10 μg/L.