Computerized Optimization of the Relative Growth of Callus Cultures of Orange Fruit Tissues7

The induction and growth of callus cultures from different parts of the fruit of Coorg mandarin orange (Citrus reticulata, Blanco) and the hormonal combinations that can maximize the growth with a basal MS medium were explored using Response Surface Methodology. The relative growth, protein, peroxidase pattern, and GC fingerprinting of volatiles were studied. Callus cultures from juice vesicles were found to be more similar to their explant counterpart than those from albedo, for all properties studied.