Open tubular capillary column with nano-crystalline cellulose derivative modified surface and application of open tubular capillary column with nano-crystalline cellulose derivative modified surface

The invention discloses an open tubular capillary column with a nano-crystalline cellulose derivative modified surface. The open tubular capillary column is prepared by the step: modifying and deriving nano-crystalline celluloses of 3,5-dimethylphenyl isocyanate on the wall of a bare capillary column subjected to activation by adopting an in-situ hybridization method. The open tubular capillary column has the advantages that the preparation is easy and a coating is stable, and can be used for capillary electrophoresis separation analysis on an amino acid chiral enantiomer.