Optimization of Ultrahigh Pressure Extraction for Salidroside From Rhodiola crenulata by Central Composite Design/Response Surface Methodology

Objective:To optimize ultrahigh pressure extraction(UPE) technology of salidroside from Rhodiola crenulata by central composite design/response surface methodology.Method:Central composite design was adopted for getting optimal ultrahigh pressure technique parameters,and compared with conventional extraction's(reflux extraction,ultrasonic extraction,microwave extraction).Result:Optimal technology conditions of UPE for salidroside from R.crenulata were:solvent was 73.3% ethanol,ratio of solvent to material(mL:g) was 29.5,extraction pressure was 255.5 mPa,extracted 1 time with 2 min.Using this extracting process,extraction yield of salidroside was 9.29 mg·g-1.It was 31.6% higher than reflux extraction,20.3% to ultrasonic extraction and 9.7% to microwave extraction.Conclusion:As a novel extraction technology of Chinese herbal medicine,UPE procedure had higher extraction yield,lower extracting temperature,required a shorter time and was less power consumption.It had provided a brand-novel method for extraction of salidroside from R.crenulata.