Integration of a plasmid into the Pseudomonas aeruginosa chromosome mediated by homologous DNA sequences

: The integrative vectors for Pseudomonas aeruginosa cells were constructed on the basis of the plasmid pSUP202. To construct the integrative vectors the fragments of chromosomal DNA mediating the homologous recombination were cloned in the plasmid. The possibility of cloning of different genes in the chromosomes of Pseudomonas aeruginosa cells was illustrated by KmR gene cloning.