Estudo do biocompósito de P3HB/nanodiamante para aplicação em acessórios médicos, parte 2: citotoxicidade in vitro e testes de biocompatibilidade

In Part 1 of this work, we presented a novel biodegradable and biocompatible nanocomposite with polymer matrix of poly-hydroxybutyrate (PHB) loaded with nanoparticles of diamonds (ND) obtained by a solvent vaporization technique for the first time, to the authors knowledge (the technique is being patented by the group) for medical devices application, its mechanical, thermal and morphological properties were investigated. The efficiency of the technique in the homogeneity and dispersion of the nanofiller in the polymer matrix was confirmed by applying mechanical models that predict these characteristics in nanocomposites. In Part 2, we tested in vitro the cytotoxicity and biocompatibility of the nanocomposite. The tests resulted in no inflammatory response of the nanocomposite in fibroblast and macrophage cell cultures for all the composition.