Studies on quality variance and genetic diversity of Gentianae Macrophyllae Radix from different habitats in Shaanxi Province.

Objective The quality variance of Gentianae Macrophyllae Radix from different habitats of Shaanxi Province was studied,the genetic diversity was detected,and the relationship between quality and inheritance was discussed.Methods Using HPLC to measure effective components of Gentianae Macrophyllae Radix from different habitats,analyze HPLC fingerprint,and calculate and compare the similarity.Based on traits of common peaks in fingerprint,different samples of Gentianae Macrophyllae Radix were clustered.The total DNA from crude medicinal materials was analyzed by RAPD,the genetic distance was calculated and different samples were clustered at the base of the genetic distance.Results There were quality variance and genetic diversity at a certain degree with Gentianae Macrophyllae Radix from different habitats of Shaanxi Province.There was no apparent correlation between quality variance and genetic diversity of Gentianae Macrophyllae Radix from different habitats of Shaanxi Province,but there was correlation between quality variance and habitats.Conclusion The habitats are more important for the quality of Gentianae Macrophyllae Radix in Shaanxi Province.This suggests that the quality of Gentianae Macrophyllae Radix is mainly affected by the environment.