Hypoxia-Induced Changes in the Bioactive of Cytotrophoblast-Derived Exosomes

s / Placenta 34 (2013) A1–A99 A80 Results: Plasma exosome concentration was increased in pregnant women compared to non-pregnant women and increased with gestational age (p < 0.05). PLAP/CD63 ratio also increased significantly (p < 0.05) during gestation. Exosmes isolated fromFT, STandTT increasedendothelial cellmigration (1.9 0.1-fold,1.6 0.2-fold and1.3 0.1-fold, respectively) compared to the control. MS analysis identified 100 different proteins involved mainly in immunomodulatory process and cell-to-cell communication. Conclusions: These data establish that the release of bioactive, PdE into the maternal plasma increases throughout pregnancy and alter the phenotype of endothelial cell in vitro. While the role of PdE in regulating maternal and/or fetal vascular responses, in normal and pathological pregnancies remains to be elucidated, changes in their protein profile may be of clinical utility in the diagnosis of placental dysfunction. http://dx.doi.org/10.1016/j.placenta.2013.06.235 P2.70. HYPOXIA-INDUCED CHANGES IN THE BIOACTIVE OF CYTOTROPHOBLAST-DERIVED EXOSOMES Carlos Salomon, Miharu Kobayashi, Murray Mitchell, Greg Rice University of Queensland Centre for Clinical Research, Brisbane/Queensland,