Effect and mechanism of allitridi against Toxoplasma gondii in vitro.

Objective To observe the effect and mechanism of allitridi against Toxoplasma gondii in vitro.Methods The Toxoplasma gondii(RH strain) and the kidney cells of rabbit were co-cultured,adding different concentration of allitridi and Sulphadiazine.The cultured cells were taken out after 24 h or 48 h,fixed and strained.The infected rate and the number of Toxoplasma gondii per parasitophorous vacuole were observed;The effect of allitridi inhibiting the proliferation of cells invaded by Toxoplasma gondii tachyzoite and normal cells were detected by MTT;The effect of allitridi inhibiting the vigor of Toxoplasma gondii tachyzoite were observed by placenta staining;And the effect of allitridi inducing the apoptosis of Toxoplasma gondii tachyzoite were measured by TUNEL.Results(1) 10～80 μg/ml allitridi could resist the infection of toxoplasma gondii in dosage-dependent way,but there wasn′t very obvious time-dependent effect;(2)With little side-effect to the cells,40 μg/ml and 80 μg/ml allitridi couldn′t inhibit the proliferation of cells;160 μg/ml allitridi had an obvious side-effect to the cells;(3) Under 80 μg/ml allitridi,the rate of Typanblue colouring was the highest,and the vigor of Toxoplasma gondii tachyzoite was the lowest;(4) Under 80 μg/ml allitridi,the rate of apoptosis of Toxoplasma gondii tachyzoite was the highest.Conclusion Allitridi could inhibit the vigor of tachyzoite,restrain its ability to infect the host′s cells and its proliferation in the cells while showing no obvious poisonous side effects.The optimal effective concentration was 80 μg/ml.Allitridi is a good medicine against Toxoplasma gondii.One of the mechanisms of its anti-Toxoplasma gondii is to induce the apoptosis of Toxoplasma gondii.