Development of a multiplex PCR for simultaneous detection of three conditioned pathogen bacteria of alimentary tract diseases

Proteus mirabilis,Salmonella and Clostridium perfringens are major conditioned pathogens causing diarrhea in animals.To simultaneous detection of three bacteria,the multiplex PCR method was established with three pairs of primers designed according to the corresponding sequences of the ureR,invA and α-toxin,respectively.The method had high specificity and sensitivity with a detection limit of 105 cfu/mL.Furthermore,the detections of tissue samples from artificially infected mice or artificially contaminated tissue samples proved that the multiplex PCR was specific and sensitive.Our data demonstrated the detection method was applicable for the differential diagnosis of P.mirabilis,Salmonella and C.perfringens infections.