Using 3D High-Content Analysis and Epigenetic Phenotyping of Cells in the Characterization of Human Prostate Tissue Heterogeneity
2015
Next-generation 3D high-content imaging and analysis enables the quantification and display of tissue heterogeneity regarding subcellular properties, and hence will improve the diagnostic characterization of biopsied tissues. ( Upper left) Needle-biopsy section of human prostate adenocarcinoma, immuno-labeled for global DNA methylation (5methylcytosine, false-colored green) and counterstained for global nuclear DNA with 4',6-diamidino-2-phenylindole (DAPI, false-colored blue) and imaged with a 20X NA=0.7 lens.
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