The polarization and proliferation of THI cells inhibited IL-2 through upregulation of SOCS-3 in naǐve CD4^＋ T cells

Objective To explore the polarization and proliferation of naǐve CD4^＋ T cells after preincubation with interleukin-2. Methods The expression of suppressor of cytokine signal （SOCS） -3 was detected by the real-time PCR in the naive T lymphocytes of DO11.10 TCR transgenic and C57BL/6N mice after they were preincubated by 50 U/ml of IL-2. The naǐve CD4^＋ T lymphocytes preincubated for 4 h by IL-2 were stimulated with ovatbumin （OVA） and inactivated BALB/c spleen cells, respectively. After 14 d, IL-12Rβ1, IL-12Rβ2 and cytoplasm IL-4 （cyIL-4） were detected by flow cytometry. Results SOCS-3 in the naǐve CD4^＋ T lympbecytes of DO11.10 TCR transgenic and C57BL/6N mice reached the peak after preincu-bated by IL-2 for 6 h. The polarization to Tn 1 and the proliferation ability of naǐve CD4^＋ T lymphocytes stimulated with specific antigen and allogeneic antigen were inhibited conspicuously during the time of the peak of SOCS-3. Conclusion The expression of SOCS-3 in naǐve CD4^＋ T lymphocytes can be upregnlated by IL-2. The upregnlation of SOCS-3 can suppress the polarization to TH1 and the proliferation ability of naǐve CD4^＋ T lympbecytes stimulated by specific antigen and allogeneic antigen. Key words: Interleukin-2 ;  Suppressor of cytokine signal-3 （ SOCS-3 ） ;  naǐve CD4^＋ T lymphocyte ;  TH1 cell