Stallion sperm integrity following centrifugation to reduce seminal plasma concentration and cool storage for 4 days

Abstract The objective of the study was to investigate if reducing the seminal plasma of stallion extended semen by centrifugation once will suffice to maintain acceptable semen quality for insemination after 4 days of cool storage. Collected semen was extended to 25 x 106 sperm/mL, non-centrifuged (Control), centrifuged for 10 min at 900 x g and 1800 x g. The supernatant partially removed, the sperm pellet reconstituted and re-extended. Placed in a passive cooling device overnight and then transferred to a refrigerator for the remainder of the cooling period. At day 0, 2 and 4, total (TM) and progressive (PM) motility, plasma (PLM) and acrosomal (ACR) membrane integrity were assessed. Centrifuged groups had higher TM and PM at day 4 compared to Control (p