2′,4′-BNA bearing a 2-pyridine nucleobase for CG base pair recognition in the parallel motif triplex DNA
2010
We succeeded in the synthesis of triplex-forming oligonucleotides (TFOs) that contain a deoxyribonucleotide (Py) bearing a 2-pyridine nucleobase or the 2′,4′-BNA congener (PyB). By UV melting experiments, it was found that 2-pyridine was a very promising nucleobase for the sequence-selective recognition of a CG base pair within double-stranded DNA (dsDNA) in a parallel motif triplex. Moreover, PyB in TFOs showed stronger affinity to a CG base pair than Py with further increase in the selectivity. Using TFO including multiple PyB units, triplex formation with dsDNA containing three CG base pairs was observed.
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