Preliminary etiological study on Acute Hemorrhagic Conjunctivitis in Beijing,2010

Objective To investigate the molecular characteristics of virus of acute hemorrhagic conjunctivitis detected in Beijing in 2010.Methods 207 conjunetival swab specimens of patients in Beijing during July and October were collected.Real-time RT-PCR method was used to type the specimens.At the same time,each specimen was,inoculated Hep-2 cells and cells were examined for the appearance of CPE.Positive cells that show CPE were detected by a real-time reverse transcription polymerase chain reaction (RT-PCR).PCR products were sequenced and analyzed.Results A total of 207 conjunctival swab specimens were detected and 19 positive CoxA24v specimens we got.No EV70 virus wag detectecd.7 positive CoxA24v PCR products were used to be sequenced.The 7 isolated strains have high homology of 95.8%～99.7%with each other.Compared with the nucleotide sequences of CoxA24v reference strain from Yunnan in 2007 and Zhejiang in 2008,the homology is 96%～99.5%and 95.7%～100%.Compared with the nucleotide sequences of CoxA24v reference strain from Shandong in 2003 and 2009,the homology is 94.3%～94.6%and 70.9%～73.4%.Phylogenetic analysis showed that the 7 isolates were located at 3 independent branch.Conclusion It was the CoxA24 variants that induced the epidemic of Acute Hemorrhagic Conjunctivitis in Beijing in 2010.And there were 3 or more individual epidemic virus strain in Beijing in 2010. Key words: Etiology; Acute Hemorrhagic Conjunctivitis; CoxA24 variant