Kitchen waste for economical amylase production using Bacillus amyloliquefaciens KCP2

Abstract: Demand of amylase in industrial sector is increasing day by day. Kitchen waste can be used for amylase production as it is generated on a daily basis and if not disposed of properly, creates various environmental problems. Bacillus amyloliquefaciens KCP2 is studied for amylase production using kitchen waste as low cost substrate under solid state fermentation. Dried kitchen waste has been used for amylase production with optimization of various physical parameters in a mineral salt medium. Maximum amylase production was obtained at 37 °C, pH 8 having 1:3 moisture ratio and 30% (v/w) inoculum after 72 h of fermentation. Amylase was partially purified by ammonium sulphate precipitation followed by dialysis with 1.2 fold purification. Partially purified enzyme showed 65 °C and 8 as optimized temperature and pH, respectively. Saccharification of 10% kitchen waste using 10 U/g enzyme showed 80% saccharification efficiency at 60 °C after 6 h.