Preparation method of molecular imprinting polymer micro-sphere with uniform size and application

The average grain diameter of a molecular imprinting polymer micro-sphere ranges from 1mum to 500mum, and grain diameter distribution coefficient C.V. is not larger than 20%. A preparation method of the molecular imprinting polymer micro-sphere includes that firstly, disperse-phase liquor is squeezed from one side of a microporous membrane into continuous-phase liquor on the other side of the microporous membrane under certain pressure, emulsion is formed, the emulsion is in cross linking under protection of nitrogen to obtain polymer micro-sphere, template molecules and single unreacted functional bodies in polymer are removed by means of washing the polymer micro-sphere, and accordingly, the molecular imprinting polymer micro-sphere with the uniform size is obtained.