Study on Anti -MuLV Effects of NCPP

Objective: To choose mice model infected by MuLV, investigate the in vivo anti - MuLV effects of NCPP and pilot evaluate its possible effects for anti - HIV. Methods:T lymphocyte transfected with MuLV(SRS8) ,were cultured with the RPMI 1640 medium added to 10% calf serum. Cells(1×105/0. 3 mL) were injected into the peritoneal cavity of mice. NCPP was divided into 3 groups, high - dose group ( 2. 0 mg per mouse)、mean - dose group (0. 5 mg per mouse) and low - dose group(0.125 mg per mouse). After 4 hours,every dose group of NCPP was given intramascular injection every other day thereafter for totally 5 times; positive control group, combivir (AZT/ 3TC) ,was given by intragastrical administration with 200 mg·kg-1·day-1 for consecutive 14 days. 14 days after the injections of NCPP and combivir( AZT/3TC ) , some mice were weighed and measured the hematology index and splenic index after killed. The others,the survival rate was figured out. Results:The 14th day after given medicine, the weight change of positive control group and mean - dose group are better than model group ( P 0. 05 ). The he matology index showed significant changes in both positive control group(combivir)and NCPP mean -dose group. The count of WBC was significant higher than that of model control group (P 0.01) and was not significant different with that of control group. NCPP mean - dose group induced significant higher splenic index than model control group and control group(P0.01). Survival rate after 30 days: NCPP High - dose/mean - dose group were significant higher than that of model control group (P0.05 ). Survival rate after 60 days: NCPP mean - dose group and High - dose/ positive control group were significant higher than that of model control group(P0.01 ,P 0. 05 ) . Conclusions: NCPP showed the significant effects in and - MuLV and significantly prolonged the survival lives of infected mice. It supposed that NCPP had the and - HIV effects in some degree.