First Report of Maize yellow mosaic virus Infecting Maize in Brazil

Maize (Zea mays) is a staple crop for human consumption and widely used as a livestock feed worldwide. Therefore, potential new diseases affecting this crop are of major concern. In May 2013, yellow mosaic and dwarfing symptoms were observed in several maize plants in a field located in the rural area of Casa Branca (21° 46′ 26″ S, 47° 5′ 9″ W), Sao Paulo state (Southeastern Brazil). Symptomatic leaves were sampled, and total RNA was isolated using Trizol™ reagent and further purified with PureLink™ RNA Mini Kit spin columns (Invitrogen, USA). A library was prepared with an Illumina TruSeq Stranded Total RNA sample preparation kit with Ribo-Zero Plant and sequenced on an Illumina Hiseq2500 platform at Macrogen Inc, South Korea. De novo assembly using Abyss 1.9 with K-mer = 64 yieldeda contig of 1183 nt with high similarities (92-93%) to several isolates sequences of Maize yellow mosaic virus (MaYMV), a putative new member of the genus Polerovirus, family Luteoviridae, recently reported in China (Chen et a...