Association of single-nucleotide polymorphisms in NAT9 and MAP3K3 genes with litter size traits in Berkshire pigs
2018
Abstract. Litter size is an economically important trait in the pig
industry. We aimed to identify genetic markers associated with litter size,
which can be used in breeding programs for improving reproductive traits.
Single-nucleotide polymorphisms (SNPs) of Berkshire pigs in the
N-acetyltransferase 9 ( NAT9 ) and Mitogen-activated protein kinase kinase kinase 3 (MAP3K3) genes were from RNA sequencing
results, and already exist in the databank (NCBI), and were confirmed by
polymerase chain reaction and restriction fragment length polymorphism
(PCR-RFLP). A total of 272 Berkshire sows were used to examine the genotype, and
their association with litter size traits was analyzed. The NAT9 SNP
was located in chromosome 12 exon 640 mRNA (A > G) and the
MAP3K3 SNP was located in chromosome 12 intron 11 (80, C > T).
Association analysis indicated that the GG genotype of
NAT9 and the CT genotype of MAP3K3 had the highest values
for litter size traits. The GG genotype expressed higher levels of
NAT9 mRNA in the endometrium than the other genotypes did, and a
positive correlation was found between litter size traits and NAT9 ,
but not MAP3K3 expression level. These results indicate that the
NAT9 and MAP3K3 can be used as candidate genes applicable
in breeding program for the improvement of litter size traits in Berkshire
pigs.
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