Identification of Glioblastoma Subpopulations by Flim

Glioblastoma multiforme (GBM) is one of the most aggressive forms of brain cancer. The tumor is known to be composed of heterogeneous subpopulations of cells, potentially playing a role in therapeutic resistance. Therefore, an understanding of the tumor heterogeneity is essential for the development of effective therapies. To further study the tumor heterogeneity, a culture method was developed to isolate distinct subpopulations from an established glioma cell line. These subpopulations include neural stem-like cells and tumor mass cells.