Purification of Flavonoids from Cordyceps militaris by Macroporous Adsorption Resin

The purification of flavonoids from Cordyceps militaris using macroporous resin was optimized by investigating several process parameters including resin type, sample volume, eluent p H, concentration and volume, and repeated use of the selected resin. Static adsorption and desorption, and kinetic studies of static adsorption demonstrated that AB-8 was selected as the better adsorbent with higher adsorption rate and adsorption capacity per unit mass for Cordyceps militarisflavonoids compared with D-101, NKA-9 and NKA-Ⅱ. As determined by optimization experiments, the recovery of flavonoids was more than 65% with a purity of over 17% when 40.0 m L of the sample(47.536 mg of flavonoids) was loaded onto a 100-m L column at a flow rate of 2 BV/h of AB-8 followed by elution with 500 m L of 85% ethanol acidified to p H 5 also at flow rate of 2 BV/h and the resin was used to repeat the process once more.