Determination of protein with surfactant and dye probe by resonance light scattering technique

It is found that sodium dodecyl sulfonate and acridine red combine with protein to form an aggregate at pH 2.10.It results in a significant enhancement of the resonance light scattering(RLS),and the enhancement degree of RLS is linear with the concentration of protein.Based on it,a method for determination of protein has been developed,different proteins and the enhancement of RLS have good linear relationship in the range of 0.030～3.0μg/mL for human serum albumin,0.20～7.0μg/mL for bovine serum albumin,the corresponding detection limits are 0.0119μg/mL和0.026μg/mL respectively.The reaction mechanism of system is discussed.The method is used for analyzing protein in human serum,and the detection results are compared with those of Coomassie brilliant blue method,there are no significant differences between two methods according to the t-test.