Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families
2011
The SMC5-6 protein complex is involved in the cellular response
to DNA damage. It is composed of 6 - 8 polypeptides, of which
Nse1, Nse3 and Nse4 form a tight sub-complex. MAGEG1, the
mammalian ortholog of Nse3, is the founding member of the MAGE
(melanoma-associated antigen) protein family and Nse4 is
related to the EID (E1A-like inhibitor of differentiation)
family of transcriptional repressors. We have identified a
conserved hydrophobic surface on the C-terminal domain of Nse3
that interacts with Nse4 and identified residues in its
N-terminal domain that are essential for interaction with Nse1.
We show that these interactions are conserved in the human
orthologs. In an examination of the evolutionary conservation
of the Nse3-Nse4 interactions, we find that most MAGE proteins
can interact with at least one of the NSE4/EID proteins.
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