Estado de la resistencia a insecticidas organosintéticos de Aedes aegypti de Coro, estado Falcon, Venezuela
2015
Aedes aegypti es una de las especies mas estudiadas en salud publica por ser vector primario en el ser humano del virus dengue, fiebre amarilla, chikungunya y zika. Se evaluo la respuesta a los insecticidas organofosforados malation, fenitrotion, pirimifos-metil al carbamato propoxur y al piretroide lambdacialotrina en una cepa de campo denominada Coro, conformada por un pool de material (inmaduro) colectado en barriadas con alta casuistica de dengue ubicadas en municipio Miranda, estado Falcon, Venezuela; en comparacion con la cepa susceptible Rockefeller. Se realizaron los bioensayos en botellas tratadas con insecticidas, segun el metodo del CDC e igualmente se identificaron los mecanismos de resistencia in vitro a traves de pruebas bioquimicas. La cepa evaluada resulto resistente a malation (45 min), fenitrotion (75 min) pirimifos-metil (75 min) con respecto a la cepa susceptible con 30, 45 y 45 min.respectivamente, con porcentajes de mortalidad en el tiempo umbral de 89,2% malation; 55,7% fenitrotion; 56,6%, pirimifos-metil. Se encontro susceptibilidad al carbamato propoxur y al piretroide lambdacialotrina. En cuanto a las pruebas bioquimicas se encontraron valores elevados para esterasas alfa (α) y beta (β) con diferencias estadisticamente significativas (P Aedes aegypti is one of the most studied species in public health as the primary vector in humans the dengue, chicunguya, zyca virus and yellow fever. Response to organophosphorus insecticides malathion, fenitrothion, methyl-pirimiphos and carbamate propoxur was evaluated and the pyrethroid lambda-field strain called Coro, made up of a pool of material (immature) collected in neighborhoods with high casuistry of dengue Coro located in Miranda municipality, Falcon state, Venezuela; compared with the susceptible Rockfeller. Bioassays in insecticide-treated bottles were performed according to the method of the CDC and also the mechanisms of resistance in vitro were identified by biochemical tests. The resistant strain was evaluated to malathion (45 min), fenitrothion (75 min) methyl-pirimiphos (75 min) compared to the susceptible strain with 30, 45 and 45 min respectively, with mortality rates of 89 time threshold, 2% malathion, fenitrothion 55.7%, 56.6%, pirimiphos-methyl. Susceptibility to propoxur carbamate and pyrethroid lambda was found. As for the high values for biochemical tests esterases alpha (α) and beta (β) with statistically significant differences (P
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