Scaffoldin Modules Serving as "Cargo" Domains To Promote the Secretion of Heterologous Cellulosomal Cellulases by Clostridium acetobutylicum†

The secretion of large heterologous cellulases by Clostridium acetobutylicum was formerly shown to be deleterious. To circumvent this issue, various scaffoldins' modules were grafted at their N termini. Family 3a cellulose binding module combined with an X2 module(s) was found to trigger the secretion of Clostridium cellulolyticum cellulases by the solventogenic bacterium.