First report of a 16SrIV-D phytoplasma associated with Texas Phoenix palm decline on pigmy date palm (Phoenix roebelenii) in Florida.

The pigmy date palm (Phoenix roebelenii O'Brien) is used as an ornamental in Florida and is popular and lucrative within the nursery trade. Severe decline of several pigmy date palms was observed at a residence in Hillsborough County, FL. Several other palm species, including P. canariensis (Canary Island date palm), P. sylvestris (wild date palm), P. dactylifera (date palm), Syagrus romanzoffiana (queen palm), and Sabal palmetto (cabbage palm), in Florida are known to be affected by Texas Phoenix palm decline (TPPD), a disease associated with a phytoplasma subgroup strain 16SrIV-D (2,3). Moreover, the location of the affected pigmy date palms was in the proximity of many other diseased cabbage palms that were identified in previous surveys and subsequently rogued. Genomic DNA was extracted from 100 mg of ground-up palm trunk tissues containing phloem cells with a DNeasy Plant Mini kit column (QIAGEN Inc., Valencia, CA) from four specimens. A high-fidelity PCR (Hf-PCR) procedure was used in preference to ...