Evaluation of recombinant LipL32 and LipL41 antigens of Leptospira interrogans serovar Canicola by ELISA for serodiagnosis of bovine leptospirosis

Recombinant LipL32 and LipL41 outer membrane proteins of Leptospira interrogans serovar Canicola were produced, and used as a pooled antigen in enzyme-linked immunosorbent assay (ELISA) to detect leptospiral antibodies in bovine sera samples. The optimum concentration of the pooled antigen was found to be 50ng of each antigen per well by using known positive and negative cattle sera. Using a total of 500 bovine sera samples the sensitivity, specificity and accuracy of pooled antigen based ELISA as compared to microscopic agglutination test (MAT) were 100%, 88.1% and 91.6%, respectively. The results suggested that antigen in ELISA could be preferred for detection of all those cases, which might have remained undiagnosed by performing MAT.