Transcriptional amplification method of nucleic acids of different temperatures gathers stages

Procedure transcriptional amplification isotherm in a single step in which: a) at least one target nucleic acid present in a biological sample is placed in the presence of the following compounds: * amplification primers * of all the necessary reagents to performing amplification, including enzymes involved in the amplification, and * at least one polyol that stabilize necessary for performing the amplification enzymes, b) denaturation of targets is performed by heating the mixture at a temperature above 44 ° c, in the presence of all the compounds c) a transcriptional target nucleic acid amplification is performed to said top temperature to 44 ° c, meeting the denaturation and amplification.