The influences of prokineticin-1 on VEGF expression in neuroblastoma QDDQ-NM cell

Objective To investigate the influence of prokineticin 1 (Prok 1)on VEGF expression in neuroblastoma cell line (QDDQ-NM).Methods Prokineticin-1 receptor gene(PKR1)small interfering RNA was transfected into neuroblastoma QDDQ NM cells.The Prok-1 and AKT protein kinase inhibitor LY294002 was respectively administered to have effect on AKT signaling pathway in cells.Western blot was conducted to detect the level of AKT-p,and the production of VEGF in supernatant was detected using ELISA assay.Results Prok-1 could activate the ATK signaling pathwayand increased AKTp level; meanwhile,the production of VEGF in QDDQNM cells was improved.Compared with control,100 ng/ml concentration of prokineticin-1 induced an increased expression of VEGF protein in post co-culture 24 h (416 ± 43 pg/ml versus 212 ± 23 pg/ml,P＜0.01 ).On the contrary,LY-294002 inhibited Prok-1 induced up-regulation of VEGF.Conclusions Prokineticin-1 increases the expression of VEGF by activating the AKT signaling pathway,through which the angiogenesis in tumor tissue is promoted and the following tumor cells proliferation and metastasis. Key words: Neuroblastoma; Vascular endothelial growth factor,endocrine-gland-derived; RNA,small interfering