Mutations of E6 and E2 oncogenes of human papillomavirus 16 in women with cervical carcinoma in Qingdao.

2010 
Objective To investigate the distribution of HPV16 E6 and E2 gene mutation in cervical cancer,and the relationship between the mutation and the development of cervical carcinoma in Qingdao,Shandong.Methods DNA samples extracted from 104 cervical cancer tissues in Qingdao were amplified by polymerase chain reaction(PCR) using specific primers for high-risk HPV,HPV16,E6 and E2 genes of HPV16,respectively.Then the PCR fragments of HPV16 E6 and E2 genes were purified,sequenced and compared with the type stain.Results The positive rates of HPV and HPV16 were 93.27%(97/104) and 69.23%(72/104) respectively.Prototype HPV16 E6 was found in 5 cases(13.51%) while mutation was detected in 32 cases(86.49%),among which the T178G or T178A(D25E) mutation was found in 25 cases(67.57%).The E2 sequence of 23 cases contained C3684A(T-K),that of 14 cases contained T3524C,C3684A(T-K) and C3787A(D-E) at the same sample,and 9 cases contained A2926G,C3159A(T-K),G3249A(R-Q),T3384C(I-T),C3410T(P-S) and C3684A(T-K) meanwhile.Conclusion There are several HPV16 E6 and E2 gene mutations in cervical cancer tissue for women in Qingdao.The mutation of E6 may relate to that of E2.
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