Antibody to Surfadant Protein A Increases Sensitivi9' of Pulmonary Surfactant to Inadivatlon by Fibrinogen in vivo

It has been suggested that surfactant protein-A (SP-A)protects surfactant activity from inhibitors such as fibrinogen. Substantial evidence indicates that inhibition of surfactant activity is often important in the pathogenesis of acute respiratory failure. Studies on surfactant function in the pulsating bubble surfactometer imply that SP-Ahelps to maintain low surface tension in the presence of inhibitors such as fibrinogen. We tested whether SP-Aacts in this way in vivo. Rabbit pups, 29 d gestational age, were treated with a monoclonal antibody to rabbit SP-A (RS) followed by fibrinogen, or with control prepa­ rations (normallgG and saline, respectively). Lungcompliance was measured during ventilation through­ out these experiments. Air-space volume and pulmonary edema were quantitated morphometrically. Animals receiving anti-SP-A antibody + fibrinogen showed substantial and significant impairment in lung compliance compared with controllittermates receiving normallgG and/or saline. Lungs from these animals showed decreased pulmonary air-space volume and increased alveolar edema. We con­ clude that SP-A protects pulmonary surfactant from inhibition by fibrinogen in vivo. This protective activity may be important in the pathogenesis of both adult and neonatal respiratory distress syndromes, and it may also be useful in devising therapies for these diseases. Strayer DS, Herting E, Sun B, Robert­ son B. Antibody to surfactant protein A Increases sensitivity of pulmonary surfactant to Inacti­ vation by fibrinogen In vivo. AM J RESPIR CRIT CARE MED 1996;153:1116-22.