Species and biotype analysis of Brucella strains isolated from cattle and sheep in Xinjiang.

[Objective] The paper aimed to understand the epidemiological characteristics of Brucella strains in Xinjiang and then provide an available integrated measure to prevent and control brucellosis. [Method] Eleven suspected Brucella strains were isolated by traditional methods,which were further identified by AMOS-PCR assay. Conventional biochemical tests were carried out to identify the biological subtype of sheep Brucella. [Result]Nine strains were all B. melitensis,and biological test indicated that all of them were B. melitensis biotype 3. [Conclusion]B. melitensis biotype 3 was the predominant strain of Brucella in Xinjiang,and AMOS-PCR assay could be applied safely and quickly as an assistant tool to detect Brucella. The results of molecular epidemiology laid a foundation for updating prevention and control strategy against brucellosis in Xinjiang.