N·Terminal Amino Acid Sequence Homologies of Group V Grass Pollen Allergens

In an earlier study, we presented data regarding the immunoaffinity purification and N-terminal sequencing of a major pollen allergen from orchard/cocksfoot grass (Dactylis glomerata), now identified as the group V allergen Dac g V. In this paper, we have extended our investigations to include group V allergens from other grass species. Our data confirm the presence of group V-restricted characteristic N-terminal amino acid sequences containing a high alanine and hydroxyproline (P′) rather than proline (P) content, and based upon two conserved elements (ADAGY and TPA/TP′A).