integrated biomass from a cell culture process for the manufacture of clear cell lysate containing plasmid DNA preparation.

Method for integrated biomass of a cell culture process for producing cell lysate clear containing plasmid DNA, characterized in that, in a first stage, filtered increasingly in a filter in the presence of an inert filter material, in preparation second stage thermally prepares the biomass contained in the filter cake and, at a later stage, the cell lysate is filtered.