Killing effects on combined treatment of citric acid and temperature for Vibrio parahaemolyticus on marine fish

The decontamination method of Vibrio parahaemolyticus was studied using response surface method(RSM).The temperature,time and pH value were selected as the single factor in the experiment.Based on the analysis of significance and interaction among different factors,the optimal condition of decontamination method was obtained as follows:temperature was 55.5℃,tims was 25s,and pH=4.96.The predicted value and measured value of death rate of Vibrio parahaemolyticus in salmon was 97.36% and 97.07%,respectively.However,there was no difference in the activity of Ca2+-ATPase in fish protein before and after treatment.