Process for the production of diacilglycerol.

Process for the production of diacylglycerol comprising the steps of: (i) reacting triacylglycerol with water to and an enzyme having a hydrolytic activity at a temperature of 20 to 90C to obtain a mixture of diacylglycerol, monoacylglycerol and free fatty acid; (Ii) removing water content in the mixture of step (i) by dehydration; and (iii) separating said monoacylglycerol, free fatty acid and residual triacylglycerol by at least one separation method to obtain a high-purity diacylglycerol; wherein the amount of water in the reaction mixture of step (i) is 20 to 180 parts by weight per 100 parts by weight of the triacylglycerol and step (i) is performed until obtaining between 5% and 50% of free fatty acids, thus producing diacylglycerol of up to 96% purity at a high yield in a short period of time.