Effects of Low-energy N+ Ion Implantation on Ethanol Fermentation Efficiency of Saccharomyces cerevisiae

[Objective] To study effects of low-energy N+ion implantation on ethanol fermentation efficiency of Saccharomyces cerevisiae.[Method]S. cerevisiae AS2.399 was selected as recipient bacterium,the optimum implantation parameter was determined,effects of ion implantation on S. cerevisiae AS 2. 399 ethanol fermentation efficiency and key enzyme expression were studied. [Result] Under the condition of implantation dose of 10 × 1015ions /cm2,the survival rate of S. cerevisiae AS 2.399 was about 25%,ethanol yield higher than that of original strain was 0. 42 g ethanol /g glucose(84% of theoretical yield),and enzyme activity values of pyruvate decarboxylase and alcohol dehydrogenase relevant to ethanol formation which was also higher than thatof original strain was approximately 0. 53 and 2. 47,respectively. [Conclusion]This report could lay significant foundation for using low-energy ion implantation technology to recombine S. cerevisiae to expand its breadth of substrate utilization and improving its efficiency of ethanol fermentation.