Biofunctionalization of nonwoven complex oriented scaffolds with distinct differentiation molecules for the directed tissue regeneration

In our research we tested electrospun scaffolds prepared from poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/polycaprolactone (PCL) with and without the vascular endothelial growth factor (VEGF) and the stromal-derived growth factor-lα (SDF-lα). Chemoattractant activity of VEGF and SDF-lα was evaluated on an endothelial cell line EA.hy 926 using in vitro migration assay. Biocompatibility of the scaffolds was assessed by implanting them into the rat pericardial sac. After 4 days of culturing, we found that the number of cells migrated to the PHBV/PCL/VEGF and PHBV/PCL/SDF-lα scaffolds was 1.4 and 1.35-fold higher, respectively, compared to the PHBV/PCL scaffolds (p < 0.05). Implantation of the scaffolds for 3 months did not cause any local or systemic inflammatory reaction. Histological examination revealed active neoangiogenesis in the PHBV/PCL/VEGF scaffolds and adjacent tissues. In addition, we detected active cell infiltration and production of extracellular matrix in the PHBV/PCL/SDF-lα scaffold...