Keratinocyte growth factor pretreatment prevents radiation-induced genetics damage in a mouse model

Objective To study the protective effect of keratinocyte growth factor (KGF) in genetics damage induced by radiation using the methods of single cell gel electrophoresis (SCGE) and micronuclei assay.Methods The C57BL/6J mice were randomly divided into five groups:control group,radiation group and low,middle,high dose groups of KGF(1,2,4 mg/kg).Drugs were administrated by intraperitoneal injection.Then control group was given sham-irradiated,and other groups were given a whole body irradiation at the dose of 7.2 Gy with γ-rays.DNA damage of different groups were detected by SCGE and micronuclei assay.Results Compared with radiation group,pretreatment with KGF can significantly decrease the tail moment and micronucleus formation.Conclusion Pretreatment with KGF can effectively protect the radiation-induced genetics damage in mouse model. Key words: Fibroblast growth factor;  Radiation injuries;  Comet assay;  Micronucleus tests