Adventitial remodeling of experimental pulmonary hypertension induces myofibroblast-produced collagen V in a Th17-pathways-related manner

Pulmonary hypertension has been focused on model for injuries of endothelial and muscular vascular layer. However, recent study showed the vascular adventitia and myofibroblast may act as regulator of vascular remodeling. Our aim is to investigate the role of myofibroblast activation in adventitial remodeling. Wister male rats were sacrificed 21d after monocrotaline treatment (MCT;n=5) compared to controls (CTR;n=5). Right ventricular pressure, morphometry of HE and picrossirius-red-staining, IHC for IL-17-related markers and “in situ” IF for SMA and collagen types I and V, and electron microscopy were used. The right ventricular pressure and cardiac hypertrophy index was higher in MCT than CTR group. Adventitial thickness to