Exploring the preparation of the brain tissue slices applying to patch clamp study

Objective To explore the preparation method of the brain tissue slices applying to patch clamp study. Methods First,the artificial cerebrospinal fluid and cutting solution should be prepared. After decollating a 2-day- old rat,its brain was transferred into a microtome,which cut the brain into slices( 300 μm). Then the brain slices were kept in an incubation chamber for 0. 5 to 1 hour. Finally,the structures of the brain tissue could be observed un- der a microscope. Results The cell activity in the brain tissue with the method above were higher. Conclusion The brain tissue can meet the needs of patch clamp research.