INHIBITION OF MULTI-TOXIC PHOSPHOLIPASE A2 OF DABOII RUSELLII PULCHELLA VENOM BY THE ETHANOLIC EXTRACT OF A. PANICULATA

Objective: Andrographis paniculata ( A. paniculata ) Nees is an important medicinal plant found in the tropical regions and has been traditionally used in Indian and Chinese medicinal systems. A. paniculata is found to exhibit anti-snake venom properties; however its inhibitory potential on multi-toxic Phospholipases A 2 and its associated inflammatory reactions is not clearly understood. Henceforth, in this study we evaluate the inhibitory/neutralizing potential of alcoholic extract of A. paniculata on the isolated multi-toxic PLA 2 (of Daboii rusellii pulchella and Naja naja ) and its induced edema in Swiss albino mice. Methods : The multi-toxic-svPLA 2 -VRV-PL-V from the venom of Daboii rusellii pulchella was purified according established methods. The inhibition of enzymatic activity and edema inducing activity was carried out according to established methods. Results : A. paniculata extract dose dependently inhibited the multi-toxic svPLA 2 enzymaticactivity with an IC 50 value of 12.4±0.6 µg/ml. Further, the extract dose dependently inhibited the edema formation, when co-injected with the enzyme indicating a strong correlation between lipolytic activity and pro-inflammatory activity. Conclusion : The ethanolic extract of A. paniculata effectively inhibited the multi-toxic svPLA 2 and its associated edema inducing activities, which substantiate their anti-ophidian properties. Further study is interesting to develop them into potent anti-ophidian agents.